The media for assisted fertilization from Medi-Cult is based on a Synthetic Serum
Replacement(SSR). The main principle of SSR is a metal iron buffer containing a balanced
mixture of iron and trace metals. Recently, Synthetic Serum Supplement(SSS) and medi-cult
are commonly used.
These synthetic substitutes have some advantages, such as safty from viral contamination
and no needs of bioassay.
To compare the usefulness between the Medi-cult and Ham's F-10 supplemented with fetal
cord serum for the culture medium of in vitro fertilization, fertinzation rate, cleavage rate and
pregnancy rate were studied. It is concluded that Medi-cult was better than Ham's F-10
supplemented with fetal cord serum according to fertilization rate, cleavage rate, pregnancy
rate and ongoning pregnancy, but that differences did not have statistical significance.
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